Research Article
Monitoring and Eliminating Pre-Analytic Laboratory Errors in an Acute Care Setup - A Case Study
Muneel Abbas Rizvi,
Zaibun Nisa*
Issue:
Volume 9, Issue 5, October 2024
Pages:
50-57
Received:
1 April 2024
Accepted:
19 April 2024
Published:
29 November 2024
Abstract: Laboratory investigations are an integral part of patient management. The number and frequency of investigations are directly related to the clinical seriousness of a patient. Hence acute care setups observe a high volume as well as frequency of sampling for laboratory analyses. Many management plans are dependent on the results of these investigations. So, there is also pressure on the pathology laboratory to provide the results as early as possible. The whole process of lab investigation requires ordering an investigation, sending the requisition/system indenture, sampling, transporting sample to the lab, analysing, result approval and sending/uploading the report. Well-planned, tried, and tested standards/ protocols must be in place in order to eliminate the probability of errors in investigation reports. Laboratories must work according to some set specifications in order to process the samples in an error free manner. This paper will analyse the procedural steps, scope of errors and steps to identify, report as well as rectify the errors in the pre-analytical phase of laboratory investigations in a highly demanding and accident-prone acute care setup. The aim of this exercise is to improve the quality of lab reporting process with an objective to eliminate pre analytic laboratory errors.
Abstract: Laboratory investigations are an integral part of patient management. The number and frequency of investigations are directly related to the clinical seriousness of a patient. Hence acute care setups observe a high volume as well as frequency of sampling for laboratory analyses. Many management plans are dependent on the results of these investigat...
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Research Article
Effects of Hinc II Polymorphism in the LDL Receptor Gene on Serum Lipid Levels of Jordanian Individuals with High Risk for Coronary Heart Disease
Nabil Ahmad Bashir*
Issue:
Volume 9, Issue 5, October 2024
Pages:
58-63
Received:
29 October 2024
Accepted:
29 November 2024
Published:
16 December 2024
Abstract: Coronary heart disease (CHD) has presented high prevalence in the Jordanian population. Nevertheless, studies of genetic risk factors for CHD in our country are insufficiently carried out. We are intended to investigated the effects of Hinc II (exon 12) polymorphisms at the low-density lipoprotein receptor (LDLR) gene on circulating lipids of 150 individuals with high risk for CHD (HRG) and 150 controls (C). Genomic DNA was extracted from white blood cells and amplified by polymerase chain reaction (PCR) and digested with HincII Restriction enzyme. LDL, TC (total cholesterol), TG (triglycerides), and HDL (high density lipoproteins) levels were measured in all subjects. RFLP (restriction fragment length polymorphism) analysis was conducted to identify genotype of LDLR gene in (HRG) and 150 controls (C). The results showed a significant correlation existed between this RFLP locus and (HRG), X2=10.6, P<0.05; H+ allele frequency: X2=7.88, P<0.05., H- allele frequency: X2=7.88, P<0.05. Genotypes H+H+ and H+H- in high risk group are significantly associated with high levels of LDL, TC, TG, P<0.05 and with low level of HDL P<0.05., while H-H- is associated with normal values of serum lipids P<0.05. It is inferred that H+ allele might be associated with high blood cholesterol level, and the H- allele with normal level. This study suggests that the differences in LDLRG genotypes might affect the phenotype of lipid metabolism.
Abstract: Coronary heart disease (CHD) has presented high prevalence in the Jordanian population. Nevertheless, studies of genetic risk factors for CHD in our country are insufficiently carried out. We are intended to investigated the effects of Hinc II (exon 12) polymorphisms at the low-density lipoprotein receptor (LDLR) gene on circulating lipids of 150 i...
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