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Occult Hepatitis C Virus in Blood Donors in Damietta - Egypt
Mohamed Ahmed Abdeldayem,
Kadry Abdelkader El-bakry,
Abdullah El-Bialy
Issue:
Volume 4, Issue 3, May 2019
Pages:
53-59
Received:
30 April 2019
Accepted:
2 June 2019
Published:
24 June 2019
Abstract: Egypt maintains the highest prevalence of hepatitis C virus infection, affecting an estimated 15%–20% of the population, new cases reported, so we search about uncommon source of hepatitis C virus infection. Occult hepatitis C Virus infection can be detected and founded in the overall public obviously haven’t any diseases, Detection of viral replication in PBMCs may increase spread and transmission of hepatitis C virus during blood transfusion, hemodialysis, and made liver disease development in occult Hepatitis C Virus infected individual. The present study aimed to detect the occult hepatitis C virus infection in healthy blood donors from Damietta-Egypt, And detection of Hepatitis C Virus core antigen. One hundred and fifty blood donors from El-Azhar University Hospital blood bank in New Damietta City were used into the present study. Detection of Hepatitis C Virus RNA in Peripheral blood mononuclear (PBMCs) and plasma by reverse transcription nested polymerase chain reaction (RT-PCR), and also detection of hepatitis C virus core antigen from plasma by ELISA. We founded that: Detection of hepatitis C virus RNA by nested PCR from PBMC among studied donors are eleven (7.9%) on the other hand Hepatitis C Virus RNA in plasma are two (1.4%) so occult C virus are 9/140 (6.4%). By comparison between results of Hepatitis C Virus core Ag and PCR evidence sensitivity 27%, specificity 98.4% in PBMC and sensitivity 100%, specificity 97.8% in Plasma.
Abstract: Egypt maintains the highest prevalence of hepatitis C virus infection, affecting an estimated 15%–20% of the population, new cases reported, so we search about uncommon source of hepatitis C virus infection. Occult hepatitis C Virus infection can be detected and founded in the overall public obviously haven’t any diseases, Detection of viral replic...
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Correlation of Albumin-Bilirubin (ALBI) Score with Child-Turcotte-Pugh (CTP) Score in the Evaluation of Liver Cirrhosis
Sabrina Shafiq,
Mohammad Nuruzzaman Khan,
Bipasha Majumder,
Mohammad Shahinul Alam,
Mohammad Saiful Islam,
Tuhin Sultana
Issue:
Volume 4, Issue 3, May 2019
Pages:
60-64
Received:
25 May 2019
Accepted:
20 June 2019
Published:
2 July 2019
Abstract: Cirrhosis of liver is characterized by the replacement of normal liver tissue by scar tissue. The Child-Turcotte-Pugh (CTP) score is used to assess the prognosis of cirrhosis and included five factors: total bilirubin level, serum albumin, prothrombin time, ascites and hepatic encephalopathy. CTP score depends on clinical assessment which may result variation in scoring. Recently a new score is introduced, Albumin-Bilirubin (ALBI) score which may be done instead of CTP scoring in cirrhotic patient. This cross sectional study was conducted at the Department of Laboratory Medicine in collaboration with the Department of Hepatology, BSMMU, Dhaka, from March 2018 to February 2019. Blood samples were assayed from eighty one diagnosed cirrhotic patients. For all statistical analysis SPSS version 22 was used. The mean age of the study group was 46.1±16.0 years. 64.2% were male and 35.8% were female. Kappa (k) value was 0.759 between ALBI and CTP score. Pearson's correlation coefficient (r) test showed significant strong positive correlation between CTP score and ALBI score (r=+0.853, p<0.001). This study concluded that the ALBI score may be done alternative to the CTP score in cirrhotic patient because it’s simple, two parameters only, more evidence based and more objective.
Abstract: Cirrhosis of liver is characterized by the replacement of normal liver tissue by scar tissue. The Child-Turcotte-Pugh (CTP) score is used to assess the prognosis of cirrhosis and included five factors: total bilirubin level, serum albumin, prothrombin time, ascites and hepatic encephalopathy. CTP score depends on clinical assessment which may resul...
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Tracheal Aspirate Cytology of 43 High-Risk Neonates to Predict Bronchopulmonary Dysplasia Early
Sahar Ali Mohamed,
Hossam Eldin Mohammed
Issue:
Volume 4, Issue 3, May 2019
Pages:
65-69
Received:
19 June 2019
Accepted:
1 July 2019
Published:
12 July 2019
Abstract: The main goal of this study was to assess the cytological evaluation of tracheal aspirate, in order to predict the early diagnosis of bronchopulmonary dysplasia (BPD) in high-risk babies during mechanical ventilation. Method: Samples from 43 infants (aged < 34 weeks and weighed ≤ 1500 g) were examined cytologically; who were admitted to Pediatric Intensive Care Units and diagnosed as respiratory distress syndrome by clinical and radiological criteria. Results: Up to 150 tracheal aspirates from 43 infants were examined to demonstrate cytological changes in bronchial epithelial cells that progressed to dysplasia. It was cytologically designated Class III. According to cytological criteria, all intubated infants had reactive hyperplastic and metaplastic epithelial changes. There were dysplastic changes in 23 out of 33 who developed BPD and 7 out of 40 who had a normal outcome. The variation between the numbers of neonates that had dysplasia in the BPD and the non-BPD group was significant (P < 0.002 by chi-square test). Conclusion: In order to monitor the development of lung injury and repair, tracheal aspirate cytology is simple, non-invasive and fast. Cytological monitoring of respiratory distress infants may be helpful in the early diagnosis and prognosis of the developing BPD. Evaluating new BPD preventive treatment schemes may also be valuable.
Abstract: The main goal of this study was to assess the cytological evaluation of tracheal aspirate, in order to predict the early diagnosis of bronchopulmonary dysplasia (BPD) in high-risk babies during mechanical ventilation. Method: Samples from 43 infants (aged ...
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