Volume 5, Issue 1, January 2020, Page: 42-46
Simultaneous Detection of 2019 Novel Coronavirus and Influenza Virus by Double Fluorescent RT-PCR
Shasha Zhang, Panjin Centers for Disease Control/Panjin Center for Inspection and Testing, Panjin, China
Xiaoli Sun, Panjin Centers for Disease Control/Panjin Center for Inspection and Testing, Panjin, China
Wei Guo, Panjin Centers for Disease Control/Panjin Center for Inspection and Testing, Panjin, China
Jianfeng Xu, Panjin Centers for Disease Control/Panjin Center for Inspection and Testing, Panjin, China
Received: Mar. 6, 2020;       Accepted: Mar. 26, 2020;       Published: Apr. 13, 2020
DOI: 10.11648/j.ajlm.20200501.16      View  152      Downloads  61
Abstract
This paper introduces a method for simultaneous detection of 2019 novel coronavirus (2019-nCoV) and influenza virus A and influenza virus B (FluA/B) by dual fluorescent RT-PCR, providing some references for the current clinical first-line practice against the epidemic. More than 12000 samples of nasopharyngeal swabs, sputum and anal swabs were collected. All samples were inactivated at 60°C for 40 min before testing, so as to protect medical examine. Nucleic acid detection kits from two manufacturers of 2019-nCoV and FluA/B were selected for the detection. Carboxyfluorescein (FAM) and green fluorescent protein (VIC) labeled probes were used to achieve simultaneous detection of the four gene targets using a double fluorescent RT-PCR reaction system. According to the results of nucleic acid detection, there is no cross infection between 2019-nCoV and FluA/B. The CT value of novel coronavirus nucleic acid in anal swab greater than CT value of sputum greater than CT value of nasopharyngeal swab in the same patient. This paper introduces a method for rapid and simultaneous detection of 2019-nCoV and FluA/B by dual fluorescent RT-PCR. It was improved the detection efficiency and reduce the cost, which could be used for rapid and emergent detection of 2019-nCoV and FluA/B. Our experiment is fast and accurate, could make reference for nucleic acid detection medical diagnosis for clinical medical staff.
Keywords
2019-nCoV, A/B Influenza Virus, Nucleic Acid Detection, Double Fluorescent RT-PCR
To cite this article
Shasha Zhang, Xiaoli Sun, Wei Guo, Jianfeng Xu, Simultaneous Detection of 2019 Novel Coronavirus and Influenza Virus by Double Fluorescent RT-PCR, American Journal of Laboratory Medicine. Vol. 5, No. 1, 2020, pp. 42-46. doi: 10.11648/j.ajlm.20200501.16
Copyright
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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