Volume 4, Issue 6, November 2019, Page: 105-110
Pre-analytical Phase in Hemostasis: The Main Anomalies and Means to Correct Them
Khayati Siham, Laboratory of Hematology, Avicenna Military Hospital, Mohammed VI UHC, Marrakech, Morocco
Mouayche Ikhlas, Laboratory of Hematology, Avicenna Military Hospital, Mohammed VI UHC, Marrakech, Morocco
Bahri Raihane, Laboratory of Hematology, Avicenna Military Hospital, Mohammed VI UHC, Marrakech, Morocco
Ait Si Ali Zineb, Laboratory of Hematology, Avicenna Military Hospital, Mohammed VI UHC, Marrakech, Morocco
Yahyaoui Hicham, Laboratory of Hematology, Avicenna Military Hospital, Mohammed VI UHC, Marrakech, Morocco
Ait Ameur Mustapha, Laboratory of Hematology, Avicenna Military Hospital, Mohammed VI UHC, Marrakech, Morocco
Chakour Mohammed, Laboratory of Hematology, Avicenna Military Hospital, Mohammed VI UHC, Marrakech, Morocco
Received: Aug. 21, 2019;       Accepted: Nov. 6, 2019;       Published: Nov. 21, 2019
DOI: 10.11648/j.ajlm.20190406.14      View  30      Downloads  11
Abstract
Pre-analytical errors still represent nearly 70% of all errors occurring in the laboratory, constituting a danger, a waste of time and an additional cost to the patient. The control of the different components of the pre-analytical step is important for the validity of the hemostasis exploration tests. The purpose of our work is to identify the main anomalies of the pre-analytical phase in hemostasis and to propose the means to correct them. We conducted a prospective and descriptive study on the pre-analytical phase of hemostasis. It was in the form of a survey, identifying the main errors related to this phase. It was performed at the Hematology laboratory of the Avicenna Military Hospital of Marrakech and spread over a period of 4 weeks. Our investigation took place at the hemostasis room, which received the collection tubes from the various hospital departments and the blood drawing room (for non-hospitalized patients). The hemostasis room received 400 prescription cards and their corresponding tubes. The parameters related to the prescription file: full name and gender of the patients, were mentioned on all the cards received and they were in conformity with those marked on the corresponding tube. The age of the patients and their clinical and therapeutic informations were mentioned in 73% and 13% of the exam requests, respectively. For the pre-analytical hemostasis parameters related to the blood collection: 63% of samples were taken at the laboratory's blood drawing room, while 37% came from the various hospital departments. Time of the realization of the samples was not mentioned on the cards or on the labels of the tubes. The anticoagulant used for all samples was sodium citrate at a concentration of 3.8%. The filling of the tubes was noncompliant in 22.25%. Registration and triage of the tubes systems were manual. Centrifugation was carried out at a rotation speed of 5000 G for 5 minutes and at a temperature set at 22°C. Hemolyzed samples accounted for 3% of the tubes.
Keywords
Hemostasis, Pre-analytical Phase, Sample
To cite this article
Khayati Siham, Mouayche Ikhlas, Bahri Raihane, Ait Si Ali Zineb, Yahyaoui Hicham, Ait Ameur Mustapha, Chakour Mohammed, Pre-analytical Phase in Hemostasis: The Main Anomalies and Means to Correct Them, American Journal of Laboratory Medicine. Vol. 4, No. 6, 2019, pp. 105-110. doi: 10.11648/j.ajlm.20190406.14
Copyright
Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Reference
[1]
Ellouze R, Guermazi S. Importance of preanalytical step in hemostasis. Annales de Biologie Clinique, Vol 71, n°4, p401-407, 2013.
[2]
Mauge L, Alhenc-Gelas M. Stability of coagulation parameters review of available data. Annales de Biologie Clinique, n°2, p 141-145, 2014.
[3]
Plebani M et al. Quality indicators to detect pre-analytical errors in laboratory testing. Clinica Chimica Acta, vol 432, p. 44-48, Mai 2014.
[4]
Gris JC. Etapes préanalytiques en hémostase. EMC – Biologie médicale, vol 6, n°3, p. 1-7, Jan 2011.
[5]
Ye Y et al. Haematology specimen acceptability: a national survey in Chinese laboratories. Biochemia Medica, vol 28, n°3, Oct 2018.
[6]
Tadesse H et al. Errors in the Hematology at St. Paul’s Hospital Millennium Medical College, Addis Ababa, Ethiopia. BMC Research Notes, vol 11, n°1, Dec 2018.
[7]
Narang V et al. Preanalytical Errors in Hematology Laboratory - an Avoidable Incompetence. Iranian Journal Of Pathology, Vol 11, n°2, p. 151-154, 2016.
[8]
Upreti S et al. Types and Frequency of Preanalytical Errors in Haematology Lab. Journal of Clinical and Diagnostic Research, Vol 7, n°11, p.. 2491-2493, Nov 2013.
[9]
Nejjar N. La phase préanalytique en hémostase: données de la littérature et enquête au laboratoire d’hématologie à l’HMIMV de Rabat. Thèse Doctorat Pharmacie, université Mohamed V faculté de médecine te de pharmacie-Rabat, n°101, 2010.
[10]
Hézard N. Hémostase pédiatrique. Bio Tribune Magazine, Vol 18, n°1, p. 40-43, 2006.
[11]
Hurtaud-Roux MF, Vincenot A, Lasne D. L’hémostase en pédiatrie, ses particularités, les principales pathologies hémorragiques et leur gestion. Anesthésie & Réanimation, vol 4, n°4, p. 290-299, 2018.
[12]
Lasne D, Hurtaud MF. Particularités de l’hémostase chez le nouveau-né. Revue Francophone des Laboratoires, n° 508, p. 72-80, Jan 2019.
[13]
Ozier Y, Cadic A, Dovergne A. Prise en charge des troubles de l’hémostase chez l’insuffisant hépatique. Transfusion Clinique et Biologique, vol 20, n°2, p. 249-254, 2013.
[14]
Fattah MA, Shaheen MH, Mahfouz MH. Disturbances of haemostasis in Diabetes Mellitus. Disease Makers, vol 19, n°6, P. 251-258, 2004.
[15]
Magnette A et al. Pre-analytical issues in the heamostasis laboratory: guidance for the clinical laboratories. Thrombosis Journal, vol 14, n° 1, Dec 2016.
[16]
Blanloeil Y et al. Effets des solutés de remplissage vasculaire sur l’hémostase. Annales Françaises d’Anesthésie et Réanimation, vol 21, n°8, p. 648-667, Oct 2002.
[17]
Linskens EA, Devreese KMJ. Pre-analytical stability of coagulation parameters in plasma stored at room temperature. International Journal of Laboratory Hematology, vol 40, n°3, p. 292-303, 2018.
[18]
Toulon P et al. Impact of different storage times at rooms temperature of unspun citrated blood samples on routine coagulation tests results. Results of a bicenter study an reviw of the literature. International Journal of Laboratory Hematology, vol 39, n°5, p. 458-468, Oct 2017.
[19]
Feng L et al. Effects of storage time and temperature on coagulation tests and factors in frensh plasma. Scientific Reports, vol 4, n° 1, May 2015.
[20]
Laboratoire de Biologie Médicale, CHU Tours. GFHT recommandations préanalytiques en hémostase, révision octobre 2015 (dernière mise à jour Mai 2017). Disponible sur: https://www.chu-tours.fr/assets/files/pdf/manuel_prelevement/LBM_M_N_DX005_02_Recommandations_pr%C3%A9-analyti.pdf.
[21]
Louati N, Ben Amor I, Daoued G. Effet de l’anticoagulant et de la conservation des échantillons sanguins sur les tests d’exploration de la coagulation. Journal de l’Information Médicale de Sfax, n°24, p. 22-23, Oct 2016.
[22]
Samama MM. Le pré-analytique en hémostase. Bio tribune magazine, n°10, p XIII, Mai 2004.
[23]
Cornes M et al. Order of blood draw: Opinion paper by the European Federation for Clinical Chemistry and laboratory Medicine (EFLM) Working Group for the Preanalytical Phase (WG-PRE). Clinical Chemistry and Laboratory Medicine (CCLM), vol 55, n°1, p. 27-31, Jan 2017.
[24]
Shukla DKB et al. Study of pre-analytical and post-analytical errors in hematology laoratory in a tertiary care hospital. Journal of Medical Science And clinical Research, vol 4, n°12, p. 14964-14967, Dec 2016.
[25]
Salvagno GL et al. Prevalence and type of pre-analytical problems for inpatients samples in coagulation laboratory. Journal of Evaluation in Clinical Practice, Vol 14, n°2, p. 351-353, 2008.
[26]
Lippi G et al. Patient and sample identification. Out of the Maze ?. Journal of Medical Biochemistry, vol 36, n°2, p. 107-112, Apr 2017.
[27]
Lippi G et al. Causes, consequences, detection and prevention of identification errors in laboratory diagnostics. Clinical Chemistry and Laboratory Medicine (CCLM), vol 47, n°2, Jan 2009.
[28]
Heyer NJ et al. Effectiveness of practices to reduce blood sample hemolysis in EDs: A laboratory medicine best practices systematic review and meta-analysis. Clinical Biochemistry, vol 45, n°13-14, p. 1012-1032, Sep 2012.
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