Volume 4, Issue 3, May 2019, Page: 53-59
Occult Hepatitis C Virus in Blood Donors in Damietta - Egypt
Mohamed Ahmed Abdeldayem, Department of Zoology, Faculty of Science, Damietta University, Damietta, Egypt
Kadry Abdelkader El-bakry, Department of Physiology, Faculty of Science Damietta University, Damietta, Egypt
Abdullah El-Bialy, Department of Medical Microbiology and Immunology, Faculty of Medicine, Zagazig University, Zagazig, Egypt
Received: Apr. 30, 2019;       Accepted: Jun. 2, 2019;       Published: Jun. 24, 2019
DOI: 10.11648/j.ajlm.20190403.11      View  103      Downloads  18
Abstract
Egypt maintains the highest prevalence of hepatitis C virus infection, affecting an estimated 15%–20% of the population, new cases reported, so we search about uncommon source of hepatitis C virus infection. Occult hepatitis C Virus infection can be detected and founded in the overall public obviously haven’t any diseases, Detection of viral replication in PBMCs may increase spread and transmission of hepatitis C virus during blood transfusion, hemodialysis, and made liver disease development in occult Hepatitis C Virus infected individual. The present study aimed to detect the occult hepatitis C virus infection in healthy blood donors from Damietta-Egypt, And detection of Hepatitis C Virus core antigen. One hundred and fifty blood donors from El-Azhar University Hospital blood bank in New Damietta City were used into the present study. Detection of Hepatitis C Virus RNA in Peripheral blood mononuclear (PBMCs) and plasma by reverse transcription nested polymerase chain reaction (RT-PCR), and also detection of hepatitis C virus core antigen from plasma by ELISA. We founded that: Detection of hepatitis C virus RNA by nested PCR from PBMC among studied donors are eleven (7.9%) on the other hand Hepatitis C Virus RNA in plasma are two (1.4%) so occult C virus are 9/140 (6.4%). By comparison between results of Hepatitis C Virus core Ag and PCR evidence sensitivity 27%, specificity 98.4% in PBMC and sensitivity 100%, specificity 97.8% in Plasma.
Keywords
Hepatitis C Virus, Occult Hepatitis C Virus, PBMCs, Hepatitis C Virus Core Ag
To cite this article
Mohamed Ahmed Abdeldayem, Kadry Abdelkader El-bakry, Abdullah El-Bialy, Occult Hepatitis C Virus in Blood Donors in Damietta - Egypt, American Journal of Laboratory Medicine. Vol. 4, No. 3, 2019, pp. 53-59. doi: 10.11648/j.ajlm.20190403.11
Copyright
Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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